Effect of light/dark cycles on the growth of Haematococcus pluvialis in mixotrophic cultivation with alternative culture media
Lúcia Helena Sipaúba-Tavares; Mayara Galatti Tedesque; Débora Cristina Fenerick; Rodrigo Ney Millan; Bruno Scardoeli-Truzzi
Abstract
Current assay investigates the influence of light/dark cycles on the growth and chemical composition of Haematoccocus pluvialis in mixotrophic cultivation and different culture media: WC, NPK and ME (macrophyte extract). Four light cycles were used: 24/0 h; 20/4 h; 16/8 h and 12/12 h light/dark cycles. Highest cell density was reported at 24/0 h and 20/4 h light/dark cycle in NPK and WC culture media, reaching 3.8 x 105 cell mL-1. Doubling time was faster in NPK culture medium with four days at 20/4 h light/dark cycle. Protein, lipid, carbon and nitrogen in the H. pluvialis biomass was higher in the absence of light in all culture media, mainly in WC culture medium at 12/12 h and 16/8 h light/dark cycles. Light/dark cycles may increase cell density and compounds synthesis, with economic benefits by saving electric power. Depending on the light cycle used the cost of the kw year-1 ranged between US$ 760 (12/12 h) and US$ 1,519 (24/0 h). NPK culture media using sugar cane molasses as carbon source with dark period is a tool to be used in H. pluvialis cultivation with cheaper cost and high nutritional values. Although WC culture medium had good results for H. pluvialis growth, however it results in an expensive cultivation.
Keywords
References
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Submitted date:
06/06/2022
Reviewed date:
09/30/2022
Accepted date:
10/15/2022
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