The downstream process choice interferes with the recovery of β-glucans of mushrooms cultured in submerged fermentation
Rafaela Caldas de Paula; Matheus Gonçalves da Costa Pereira; Crystian Bartsch Parodi; Pedro Cazetta da Cruz; Luiz Fernando Bianchini; Edvaldo Antonio Ribeiro Rosa
Abstract
Mushroom β-glucans (βG) have several health-benefiting properties, but the production and extraction of these polysaccharides need to be improved. This study aimed to identify species of edible/medicinal basidiomycetes that produce greater biomass in submerged fermentation and determine the most effective extraction method for those with the best yield. Ganoderma lucidum, Schizophyllum commune, Trametes versicolor, and Pleurotus eryngii were cultivated in nutrient broth for 120 h. The extraction of βG was done by boiling in ethanol and sodium hydroxide. Only S. commune and T. versicolor presented polysaccharide levels, showing greater biomass. Extraction with NaOH was more effective for T. versicolor, while ethanol was better for S. commune. The study concluded that the extraction of βG must be customized for each strain, and the yield of the conventional enzymatic extraction method was comparable to that obtained.
Keywords
References
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Submitted date:
10/30/2024
Accepted date:
12/19/2024
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