In vitro activity of free and nanostructured essential oils of Ocimum basilicum, Cymbopogon citratus and Eucalyptus globulus in Fasciola hepatica eggs
Bruna Baccega, Alexia Brauner de Mello, Yan Wahast Islabão, Maria Antonieta Silva, Aline Ferreira Ourique, Samanta da Silva Gündel, Angelita Milech, Camila Belmonte Oliveira
Abstract
Fasciolosis is one of the most important parasitic diseases in animal health, primarily affecting ruminants and causing economic and productivity losses. This study aimed to evaluate the in vitro ovicidal activity of essential oils from Ocimum basilicum (Basil), Cymbopogon citratus (Lemongrass), and Eucalyptus globulus and their respective nanostructures in Fasciola hepatica eggs at concentrations of 0.5, 1, and 1.5%. The eggs were deposited in six-well plates along with the free and nanostructured essential oils at their respective concentrations, packaged in aluminum paper, and incubated at 27°C and 80% humidity in a B.O.D. for 14 days. As a positive control, therapeutic dose albendazole (0.025 mg) was used. For negative control eggs only and water, blank nanostructure was used to control the nanostructured samples. Egg counts for hatched and total eggs were performed at 14 and 21 days using an inverted microscope. For the tests conducted with free and nanostructured essential oils of lemongrass and basil, better results were obtained in all tested concentrations. However, neither free nor nanostructured eucalyptus oil showed proven efficacy against F. hepatica eggs in this study.
Keywords
References
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Submitted date:
06/02/2025
Accepted date:
12/03/2025
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