Microbial fuel cells potential of marine actinobacteria Actinoalloteichus sp. MHA15 from the Havelock island of the Andamans, India
Rajagopal Gobalakrishnan, Rameshbabu Bhuvaneswari
Abstract
In the recent past, use of fossil fuel is on the rise and has triggered global energy crisis. So, renewable bioenergy is viewed as one of the means to tackle this problem. In this context, interest in microbial fuel cells (MFCs) is increasing and gaining popularity due to their ability to convert the organic wastes into renewable energy. Present investigation is on the bioelectricity production by a marine actinobacterium isolated from the Havelock island of the Andamans. Actinobacterial colonies were isolated from the sediment samples, using Kuster's agar. 19 morphologically distinct strains were subjected to cellulase enzyme screening. Among them, higher cellulose degradation capacity was found in the strain MHA15. This potential strain was selected and identified as a species close to Actinoalloteichus cyanogriseus. The strain was subjected to bioelectricity generation using sugarcane bagasse as substrate and was evaluated in a dual chambered microbial fuel cell. In the MFC, initial voltage output started at 160 mV and it gradually increased, reaching a maximum of 257 mV at the 3rd hour in the actinobacterial incubated bagasse solution. From the MFC analyzed sludge bagasse, microbial diversity was searched using different media and only actinobacterial colonies were observed. Conventional taxonomic characters of the isolates were identical to the potential actinobacterial strain MHA15 which produced bioelectricity from bagasse. Marine actinobacteria, with their unique nature, differ very much in many aspects from their terrestrial counterparts and are known to produce diverse spectra of novel and useful substances and excellent bioactivity. Results of the present study have ascertained that the marine actinobacterial strain Actinoalloteichus sp. MHA15 is capable of generating bioelectricity and there is much scope for utilizing such marine actinobacteria for large scale production of bioelectricity, after further in-depth studies.
Keywords
References
Ahmed et al., 2014
O.B. Ahmed, A.H. Asghar, M.M. Elhassan
Comparison of three DNA extraction methods for polymerase chain reaction (PCR) analysis of bacterial genomic DNA
African Journal of Microbiology Research, 8 (6) (2014), pp. 598-602
Alam et al., 2004
M.Z. Alam, M.A. Manchur, M.N. Anwar
Isolation, purification, characterization of cellulolytic enzymes produced by the isolate Streptomyces omiyaensis
Pakistan Journal of Biological Sciences, 7 (10) (2004), pp. 1647-1653
Anderson and Wellington, 2001
A.S. Anderson, M.H.E. Wellington
The taxonomy of Streptomyces and related genera
International Journal of Systematic and Evolutionary Microbiology, 51 (3) (2001), pp. 797-814
Bailey and Ollis, 1986
J.E. Bailey, F.D. Ollis
Biochemical engineering fundamentals
(2nd ed.), McGraw-Hill, New York (1986)
Biffinger et al., 2008
J.C. Biffinger, J.N. Byrd, B.L. Dudley, B.R. Ringeisen
Oxygen exposure promotes fuel diversity for Shewanella oneidensis microbial fuel cells
Biosensors and Bioelectronics, 23 (6) (2008), pp. 820-826
Call and Logan, 2008
D. Call, B.E. Logan
Hydrogen production in a single chamber microbial electrolysis cell lacking a membrane
Environmental Science and Technology, 42 (9) (2008), pp. 3401-3406
Candido et al., 2012
R.G. Candido, G.G. Godoy, A.R. Goncalves
Study of sugarcane bagasse pretreatment with sulfuric acid as a step of cellulose obtaining
World Academy of Science, Engineering and Technology, 61 (2012), pp. 101-105
Chae et al., 2008
K.J. Chae, M. Choi, F. Ajayi, W. Park
Mass transport through a proton exchange membrane (Nafion) in microbial fuel cells
Energy and Fuel, 22 (1) (2008), pp. 169-176
Cummins and Harris, 1958
C.S. Cummins, H. Harris
Studies on the cell-wall composition and taxonomy of Actinomycetales and related groups
Microbiology, 18 (1) (1958), pp. 173-189
Davis and Higson, 2007
F. Davis, S.P. Higson
Biofuel cells – recent advances and applications
Biosensors and Bioelectronics, 22 (7) (2007), pp. 1224-1235
Dutta et al., 2008
T. Dutta, R. Sahoo, R. Sengupta, S.S. Ray, A. Bhattacharjee, S. Ghosh
Novel cellulases from an extremophilic filamentous fungi Penicillium citrinum: Production and characterization
Journal of Industrial Microbiology and Biotechnology, 35 (4) (2008), pp. 275-282
Felsenstein, 1985
J. Felsenstein
Confidence limits on phylogenies: An approach using the bootstrap
Evolution, 39 (4) (1985), pp. 783-791
Gobalakrishnan and Sivakumar, 2017
R. Gobalakrishnan, K. Sivakumar
Systematic characterization of potential cellulolytic marine actinobacteria Actinoalloteichus sp MHA15
Biotechnology Reports, 13 (2017), pp. 30-36
Gobalakrishnan et al., 2016
R. Gobalakrishnan, G. Radha, K. Sivakumar, Naresh, R.R. Rashmi, L. Kannan
Screening of industrially important enzymes of potential marine actinobacteria of the Neil Island, the Andamans, India
Journal of Bioresources, 3 (1) (2016), pp. 20-30
Haandel and Lettanga, 1994
A.C.V. Haandel, G. Lettanga
Anaerobic sewage treatment
John Wiley and Sons Chichester, UK (1994)
Hall, 1999
T.A. Hall, BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT
Nucleic Acids Symposium Series, 41 (1999), pp. 95-98
Hardter et al., 2012
U. Hardter, M. Luzhetska, S. Ebeling, A. Bechthold
Ethanol production in actinomycetes after expression of synthetic adhB and pdc
The Open Biotechnology Journal, 6 (2012), pp. 13-16
Jadhav and Ghangrekar, 2009
G.S. Jadhav, M.M. Ghangrekar
Performance of microbial fuel cell subjected to variation in pH temperature external load and substrate concentration
Bioresource Technology, 100 (2) (2009), pp. 717-723
Karuppiah et al., 2011
V. Karuppiah, C. Aarthi, K. Sivakumar
Enhancement of PCR amplification of actinobacterial 16S rRNA gene using an adjuvant, dimethyl sulphoxide
Current Science, 101 (1) (2011), pp. 22-23
Kim et al., 2008
I.S. Kim, K.J. Chae, M.J. Choi, W. Verstraete
Microbial fuel cells: Recent advances, bacterial communities and application beyond electricity generation
Environmental Engineering Research, 13 (2) (2008), pp. 51-65
Kumar et al., 2013
R. Kumar, S.A. Lone, S. Ali, S. Pattnaik, N. Kumari, D. Kumar, P. Nehra, P. Seenivasan
Cellulolytic activity of actinomycetes isolated from Areraj region Bihar
Current Discovery, 2 (1) (2013), pp. 92-96
Kuster and Williams, 1964
E. Kuster, S.T. Williams
Production of hydrogen sulphide by Streptomyces and methods for its detection
Applied Microbiology, 12 (1) (1964), pp. 46-52
Larrosa-Guerrero et al., 2010
A. Larrosa-Guerrero, K. Scott, I.M. Head, F. Mateo, A. Ginesta, C. Godinez
Effect of temperature on the performance of microbial fuel cells
Fuel, 89 (12) (2010), pp. 3985-3994
Lechevalier and Lechevalier, 1970
M.P. Lechevalier, H. Lechevalier
Chemical composition as a criterion in the classification of aerobic actinomycetes
International Journal of Systematic Bacteriology, 20 (4) (1970), pp. 435-443
Lee et al., 2008
Y.J. Lee, B.K. Kim, B.H. Lee, K.I. Jo, N.K. Lee, C.H. Chung, Y.C. Lee, J.W. Lee
Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull
Bioresource Technology, 99 (2) (2008), pp. 378-386
Lee et al., 2014
L.H. Lee, N. Zainal, A.S. Azman, S.K. Eng, B.H. Goh, W.F. Yin, N.S.A. Mutalib, K.G. Chan
Diversity and antimicrobial activities of actinobacteria isolated from tropical mangrove sediments in Malaysia
Scientific World Journal (698178) (2014), pp. 1-14
Lemmer and Baumann, 1988
H. Lemmer, M. Baumann
Scum actinomycetes in sewage treatment plants – Part 3: Synergisms with other sludge bacteria
Water Research, 22 (6) (1988), pp. 765-767
Li and Yu, 2014
W.W. Li, H.Q. Yu
He Z. Towards sustainable wastewater treatment by using microbial fuel cells-centered technologies
Energy and Environmental Science, 7 (3) (2014), pp. 911-924
Lin and Tanaka, 2005
Y. Lin, S. Tanaka
Ethanol fermentation from biomass resources: Current state and prospects
Applied Microbiology and Biotechnology, 69 (6) (2005), pp. 627-642
Liu and Cheng, 2014
W.F. Liu, S. Cheng
Microbial fuel cells for energy production from wastewaters: The way toward practical application
Applied Physics and Engineering, 15 (11) (2014), pp. 841-861
Liu et al., 2009
Z. Liu, J. Liu, S. Zhang, Z. Su
Study of operational performance and electrical response on mediatorless microbial fuel cells fed with carbon and protein rich substrates
Biochemical Engineering Journal, 45 (3) (2009), pp. 185-191
Logan et al., 2015
B.E. Logan, J.W. Maxwell, K.Y. Kim, W. He, Y. Feng, E.S. Pascal
Assessment of microbial fuel cell configurations and power densities
Environmental Science and Technology Letters, 2 (2015), pp. 206-214
Lovley, 2006a
D.R. Lovley
Microbial fuel cells: Novel microbial physiologies and engineering approaches
Current Opinion in Biotechnology, 17 (3) (2006), pp. 327-332
Lovley, 2006b
D.R. Lovley
Bug juice: Harvesting electricity with micro-organisms
Nature Reviews Microbiology, 4 (7) (2006), pp. 497-508
Lovley, 2008
D.R. Lovley
The microbe electric: Conversion of organic matter to electricity
Current Opinion in Biotechnology, 19 (6) (2008), pp. 564-571
Mathis et al., 2008
B.J. Mathis, C.W. Marshall, C.E. Milliken, R.S. Makkar, S.E. Creager, H.D. May
Electricity generation by thermophilic microorganisms from marine sediment
Applied Microbiology and Biotechnology, 78 (1) (2008), pp. 147-155
Mathuriya and Yakhmi, 2014
A.S. Mathuriya, J.V. Yakhmi
Microbial fuel cells to recover heavy metals
Environmental Chemistry Letters, 12 (4) (2014), pp. 483-494
Mohanraj et al., 2011
D. Mohanraj, S. Bharathi, M. Radhakrishnan, R. Balagurunathan
Bioprospecting of actinobacteria from Yelagiri hills with special reference to antibacterial activity
Journal of Chemical and Pharmaceutical Research, 3 (3) (2011), pp. 439-446
Muralidharan et al., 2011
A. Muralidharan, O.K. Ajay Babu, K. Nirmalraman, M. Ramya
Impact of salt concentration on electricity production in microbial hydrogen based salt bridge fuel cells
Indian Journal of Fundamental and Applied Life Sciences, 1 (2) (2011), pp. 178-184
Mustakeem, 2015
Mustakeem
Electrode materials for microbial fuel cells: Nanomaterial approach
Materials for Renewable and Sustainable Energy, 4 (22) (2015), pp. 1-11
Nevin et al., 2008
K.P. Nevin, H. Richter, S.F. Covalla, J.P. Johnson, T.L. Woodard, A.L. Orloff, H. Jia, M. Zhang, D.R. Lovley
Power output and columbic efficiencies from biofilms of Geobacter sulfurreducens comparable to mixed community microbial fuel cells
Environmental Microbiology, 10 (10) (2008), pp. 2505-2514
Niessen et al., 2005
J. Niessen, U. Schroder, F. Harnisch, F. Scholz
Gaining electricity from in situ oxidation of hydrogen produced by fermentative cellulose degradation
Letters in Applied Microbiology, 41 (3) (2005), pp. 286-290
Nonomura, 1974
H. Nonomura
Key for classification and identification of 458 species of the Streptomycetes included in ISP
Journal of Fermentation Technology, 52 (2) (1974), pp. 78-92
Pandey et al., 2000
A. Pandey, P. Nigam, C.R. Soccol, V.T. Soccol, D. Singh, R. Mohan
Advances in microbial amylases
Biotechnology and Applied Biochemistry, 31 (2) (2000), pp. 135-152
Pant et al., 2010
D. Pant, G.V. Bogaert, L. Diels, K. Vanbroekhoven
A review of the substrates used in Microbial Fuel Cells (MFCs) for sustainable energy production
Bioresource Technology, 101 (6) (2010), pp. 1533-1543
Park and Zeikus, 2000
D.H. Park, J.G. Zeikus
Electricity generation in microbial fuel cells using neutral red as an electronophore
Applied and Environmental Microbiology, 66 (4) (2000), pp. 1292-1297
Raghavulu et al., 2009
S.V. Raghavulu, S. Venkata Mohan, R.M. Venkateswar, G. Mohanakrishna, P.N. Sarma
Behavior of single chambered mediatorless microbial fuel cell (MFC) at acidophilic neutral and alkaline microenvironments during chemical wastewater treatment
International Journal of Hydrogen Energy, 34 (17) (2009), pp. 7547-7554
Rahimnejad et al., 2015
M. Rahimnejad, A. Adhami, S. Darvari, A. Zirepour, S.E. Oh
Microbial fuel cell as new technology for bioelectricity generation: A review
Alexandria Engine Journal, 54 (3) (2015), pp. 745-756
Ramachandran et al., 2015
R. Ramachandran, S.M. Chen, G.P. Gnanakumar
Enhancement of different fabricated electrode materials for microbial fuel cell applications an overview
International Journal of Electrochemical Science, 10 (2015), pp. 7111-7137
Rathnan and Ambili, 2011
R.K. Rathnan, M. Ambili
Cellulase enzyme production by Streptomyces sp using fruit waste as substrate
Australian Journal of Basic and Applied Sciences, 5 (12) (2011), pp. 1114-1118
Reddy et al., 2010
L.V. Reddy, S. Pradeep Kumar, Y.J. Wee
Microbial Fuel Cells (MFCs) – A novel source of energy for new millennium
Current Research Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 2 (2010)
Ren et al., 2007
Z. Ren, E.T. Ward, J.M. Regan
Electricity production from cellulose in a microbial fuel cell using a defined binary culture
Environmental Science and Technology, 41 (13) (2007), pp. 4781-4786
Rezaei et al., 2008
F. Rezaei, T.L. Richard, B.E. Logan
Enzymatic hydrolysis of cellulose coupled with electricity generation in a Microbial Fuel Cell
Biotechnology and Bioengineering, 101 (6) (2008), pp. 1163-1169
Rezaei et al., 2009
F. Rezaei, D. Xing, R. Wagner, J.M. Regan, T.L. Richard, B.E. Logan
Simultaneous cellulose degradation and electricity production by Enterobacter cloacae in a microbial fuel cell
Applied and Environment Microbiology, 75 (11) (2009), pp. 3673-3678
Rismani-Yazdi et al., 2007
H. Rismani-Yazdi, A.D. Christy, B.A. Dehority, M. Morrison, Z. Yu, O.H. Tuovinen
Electricity generation from cellulose by rumen microorganisms in microbial fuel cells
Biotechnology and Bioengineering, 97 (6) (2007), pp. 1398-1407
Rozendal et al., 2006
R.A. Rozendal, H.V. Hamelers, C.J. Buisman
Effects of membrane cation transport on pH and microbial fuel cell performance
Environmental Science and Technology, 40 (17) (2006), pp. 5206-5211
Saitou and Nei, 1987
N. Saitou, M. Nei
The neighbor-joining method: A new method for reconstructing phylogenetic trees
Molecular Biology and Evolution, 4 (4) (1987), pp. 406-425
Sarranyadhevi et al., 2014
D. Sarranyadhevi, T. Shanmugasundaram, J. Thirumalairaj, R. Balagurunathan
Microbial fuel cells an actinobacterial mediated novel approach for power generation
Journal of Current Perspectives in Applied Microbiology, 3 (2) (2014), pp. 45-53
Schroder, 2007
U. Schroder
Anodic electron transfer mechanisms in microbial fuel cells and their energy efficiency
Physical Chemistry Chemical Physics, 9 (21) (2007), pp. 2619-2629
Schroder et al., 2003
U. Schroder, J. Niessen, F. Scholz
A generation of microbial fuel cells with current outputs boosted by more than one order of magnitude
Angewandte Chemie International Edition, 42 (25) (2003), pp. 2880-2883
Scott and Murano, 2007
K. Scott, C. Murano
A study of a microbial fuel cell battery using manure sludge waste
Journal of Chemical Technology and Biotechnology, 82 (9) (2007), pp. 809-817
Sethubathi, 2011
G.V.B. Sethubathi
Evaluation of marine actinobacteria from the coral reef environment of the Little Andaman Island (India) for Microbial Fuel Cell potential
PhD Thesis
Annamalai University, India (2011)
Sharma and Li, 2010
Y. Sharma, B. Li
Optimizing energy harvest in wastewater treatment by combining anaerobic hydrogen producing biofermentor (HPB) and microbial fuel cell (MFC)
International Journal of Hydrogen Energy, 35 (8) (2010), pp. 3789-3797
Shirling and Gottlieb, 1966
E.B. Shirling, D. Gottlieb
Methods for characterization of Streptomyces species
International Journal of Systematic Bacteriology, 16 (3) (1966), pp. 313-340
Sivakumar et al., 2015
L. Sivakumar, H. Ann Suji, L. Kannan
Bioelectricity production by marine bacteria, actinobacteria, and fungi
Marine Bioenergy Trends and Developments (2015), pp. 515-526
Soddell and Seviour, 1998
J.A. Soddell, R.J. Seviour
Numerical taxonomy of Skermania piniformis and related isolates from activated sludge
Journal of Applied Microbiology, 84 (2) (1998), pp. 272-284
Tamura et al., 2000
T. Tamura, L. Zhiheng, Z. Yamei, K. Hatano
Actinoalloteichus cyanogriseus gen. nov.,sp. nov
International Journal of Systematic and Evolutionary Microbiology, 50 (Pt4) (2000), pp. 1435-1440
Tamura et al., 2011
K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar
MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
Molecular Biology and Evolution, 28 (10) (2011), pp. 2731-2739
Venkata Mohan et al., 2010
S. Venkata Mohan, G. Mohanakrishna, P.N. Sarma
Composite vegetable waste as renewable resource for bioelectricity generation through non-catalyzed open-air cathode microbial fuel cell
Bioresource Technology, 101 (3) (2010), pp. 970-976
Zhou et al., 2011
M. Zhou, M. Chi, J. Luo, H. He, T. Jin
An overview of electrode materials in microbial fuel cells
Journal of Power Sources, 196 (10) (2011), pp. 4427-4435
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