Response with TH1 profile obtained in vaccine formulation against Caseous Lymphadenitis in animal model C57 Black/6
Daniela Droppa-Almeida, Wanessa L. P. Vivas, Ricardo Evangelista Fraga, Andrea F. S. Rezende, Lumar Lucena Alves, Roberto Meyer, Isabel B. Lima-Verde, Odir Delagostin, Sibele Borsuk, Francine F. Padilha
Abstract
Caseous Lymphadenitis (CLA) is a contagious disease that compromises the quality of life of sheep and goats. Caused by Corynebacterium pseudotuberculosis CLA is responsible for considerable economic losses in sheep and goat farming. Therefore, the search for preventive measures, such as the development of vaccines is increasing. To evaluate the immunoprotective response of experimental vaccines different murine models susceptible to infections are used. Thus, the objective of this study was to evaluate the protective potential of the recombinant subunit vaccine using an endoglycosidase (rCP40) of C. pseudotuberculosis associated with Saponin and Complete Freund adjuvant (CFA) in murine model C57/Black6. Thus, four groups of animals were separated, where G1 and G2 were control groups and G3 and G4 were experimental groups (rCP40 + Saponin) and (rCP40 + CFA) respectively. The evaluation of the production of reactive antibodies to rCP40 showed that the animals inoculated with the adjuvants presented potentiation of the cellular and humoral immune response, presenting higher production of IgG2a and IgG2b. After the challenge, only the control groups died, while in the experimental groups, although some survived, they presented granulomas, which are characteristics of CLA.
Keywords
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