Effects of Avicennia marina extracts on Labeo rohita (Ham) challenged with Pseudomonas fluorescens
G. Dinesh Kumar, M. Karthik, R. Rajakumar, K. Baskar
Abstract
The medicinal plant Avicennia marina was evaluated for their immunostimulatory activity on Pseudomonas fluorescens infested fish, Labeo rohita. The fish was dosed intraperitoneally at 10, 20 and 30 ppm concentrations of ethanolic leaves extract of A. marina and control. After 10, 20 and 30 days of treatments, the immunological, hematological and serum protein level of fish was assessed in control and treatments. All the concentration of plant leaves extract significantly enhanced the agglutination, hematological parameters and total serum protein on 30th days after treatment. The highest agglutination activity was observed in the group treated with 30 ppm concentration of A. marina on 20 days. The WBC, RBC and hemoglobin content was increased with increasing concentration of the treatments. The results, clearly indicates that A. marina leaves extract will be used as immunostimulatory agent to aquaculture for mass production of healthy fish.
Keywords
References
Aathi et al., 2013
K. Aathi, V. Ramasubramanian, V. Uthayakumar, S. Munirasu
Effect of chitosan supplementary diet on survival, growth, haematological, biochemical, and immunological response of Indian major carp Lobeo rohita
International Research Journal of Pharmacy, 4 (5) (2013), pp. 141-147, 10.7897/2230-8407.04529
Alam et al., 2016
M.E. Alam, M.M.M. Hossain, S.B. Bappa, B.K. Dey, M. Hasan-Uj-Jaman, S. Khondoker, et al.
Achyranthes aspera extract as feed additives enhances immunological parameters and growth performance in Pangasius pangasius against Pseudomonas fluorescens
International Journal of Fisheries and Aquatic Studies, 4 (3) (2016), pp. 193-198
http://www.fisheriesjournal.com/archives/2016/vol4issue3/PartC/4-2-38.pdf
Bandaranayake, 1998
W.M. Bandaranayake
Traditional and medicinal uses of mangroves
Mangroves and Salt Marshes, 2 (3) (1998), pp. 133-148, 10.1023/A:1009988607044
Bradford, 1976
M.M. Bradford
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Analytical Biochemistry, 72 (1–2) (1976), pp. 248-254, 10.1016/0003-2697(76)90527-3
Citarasu, 2010
T. Citarasu
Herbal biomedicines: A new opportunity for aquaculture industry
Aquaculture International, 18 (3) (2010), pp. 403-414, 10.1007/s10499-009-9253-7
Das et al., 2009
B.K. Das, J. Pradhan, S. Sahu
The effect of Euglena viridis on immune response of rohu Labeo rohita (Ham.)
Fish and Shellfish Immunology, 26 (6) (2009), pp. 871-876, 10.1016/j.fsi.2009.03.016
Das et al., 2013
R. Das, R.P. Raman, H. Saha, R. Singh
Effect of Ocimum sanctum Linn (Tulsi) extract on the immunity and survival of Labeo rohita (Hamilton) infected with Aeromonas hydrophila
Aquaculture Research, 43 (5) (2013), pp. 1-11, 10.1111/are.12264
Dineshkumar et al., 2014
G. Dineshkumar, R. Rajakumar, P. Mani
Immunostimulant effect on Eichhornia crassipes (Mart.) Solms against P. fluorescens infected Indian major carp Labeo rohita (HAM.)
European Journal of Molecular Biology and Biochemistry, 1 (5) (2014), pp. 158-164
http://mcmed.us/downloads/141066321964.pdf
Drabkin, 1946
D.L. Drabkin
Spectrophotometric studies. XIV. The crystallographic and optical properties of the hemoglobin of man in comparison with those of other species
The Journal of Biological Chemistry, 164 (2) (1946), pp. 703-709
Elliott and Shotts, 1980
D.G. Elliott, E.B. Shotts Jr.
Aetiology of an ulcerative disease in goldfish, Carassius auratus (L.): Experimental induction of the disease
Journal of Fish Diseases, 3 (2) (1980), pp. 145-151, 10.1111/j.1365-2761.1980.tb00197.x
Gobi et al., 2016
N. Gobi, C. Ramya, B. Vaseeharan, B. Malaikozhundan, S. Vijayakumar, K. Murugan, et al.
Oreochromis mossambicus diet supplementation with Psidium guajava leaf extracts enhance growth, immune, antioxidant response and resistance to Aeromonas hydrophila
Fish and Shellfish Immunology, 58 (2016), pp. 572-583, 10.1016/j.fsi.2016.09.062
Karunasagar et al., 1997
I. Karunasagar, A. Ali, S.K. Otta
Immunization with bacterial antigens: Infections with motile aeromonads
Developments in Biological Standardization, 90 (1997), pp. 135-141
https://www.ncbi.nlm.nih.gov/pubmed/9270842
Kathiresan and Bingham, 2001
K. Kathiresan, B.L. Bingham
Biology of mangroves and mangrove ecosystems
Advances in Marine Biology, 40 (2001), pp. 81-251, 10.1016/S0065-2881(01)40003-4
Lee et al., 2017
N.Y. Lee, M.A.C. Yunus, Z. Idham, M.S.H. Ruslan, A.H.A. Aziz, N. Irwansyah
Extraction and identification of bioactive compounds from agarwood leaves
IOP Conference Series: Materials Science and Engineering, 162 (2017), p. 012028, 10.1088/1757-899X/162/1/012028
Logambal et al., 2000
S.M. Logambal, S. Venkatalakshmi, R.D. Micheal
Immunostimulatory effect of leaf extract of Ocimum santum Linn. in Oreochromis mossambicus (Peters)
Hydrobiologia, 430 (1–3) (2000), pp. 113-120, 10.1023/A:1004029332114
Michell et al., 1970
R.H. Michell, M.J. Karnovsky, M.L. Karnovsky
The distributions of some granule-associated enzymes in guinea-pig polymorphonuclear leucocytes
Biochemical Journal, 116 (2) (1970), pp. 207-216, 10.1042/bj1160207
Ngugi et al., 2015
C.C. Ngugi, E. Oyoo-Okoth, J. Mugo-Bundi, P.S. Orina, E.J. Chemoiwa, P.A. Aloo
Effects of dietary administration of stinging nettle (Urticadioica) on the growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) challenged with Aeromonas hydrophila
Fish and Shellfish Immunology, 44 (2) (2015), pp. 533-541, 10.1016/j.fsi.2015.03.025
Nugroho et al., 2017
R.A. Nugroho, H. Manurung, F.M. Nur, W. Prahastika
Terminalia catappa L. extract improves survival, haematological profile and resistance to Aeromonas hydrophila in Betta sp.
Archives of Polish Fisheries, 25 (2017), pp. 103-115, 10.1515/aopf-2017-0010
Robertsen, 1999
B. Robertsen
Modulation of the non-specific defence of fish by structurally conserved microbial polymers
Fish & Shellfish Immunology, 9 (4) (1999), pp. 269-290, 10.1006/fsim.1998.0186
Russia and Stood, 1992
V. Russia, S.K. Stood
Procedure manual for routine diagnostic tests
K.L. Mukherjee, S. Ghosh (Eds.), Medical laboratory technology, Vol. 3, Tata Mcgraw Hill Publishing Company, New Delhi, India (1992), pp. 252-258
Sahu et al., 2007a
S. Sahu, B.K. Das, B.K. Mishra, J. Pradhan, N. Sarangi
Effect of Allium sativum on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila
Journal of Applied Ichthyology, 23 (1) (2007), pp. 80-86, 10.1111/j.1439-0426.2006.00785.x
Sahu et al., 2007b
S. Sahu, B.K. Das, J. Pradhan, B.C. Mohapatra, B.K. Mishra, N. Sarangi
Effect of Magnifera indica kernel as a feed additive on immunity and resistance to Aeromonas hydrophila in Labeor ohita fingerlings
Fish and Shellfish Immunology, 23 (1) (2007), pp. 109-118
Talpur et al., 2013
A.D. Talpur, M. Ikhwanuddin, A.M.A. Bolong
Nutritional effects of ginger (Zingiber officinale Roscoe) on immune response of Asian sea bass, Lates calcarifer (Bloch) and disease resistance against Vibrio harveyi
Aquaculture (2013), 10.1016/j.aquaculture.2013.02.043
400–401, 46–52
Uluköy et al., 2018
G. Uluköy, A. Kubilay, B.I. Didinen, S. Metin, S. Altun, O. Diler, et al.
Immunostimulant effects of geophyte plant extract on non-specific defense mechanisms of rainbow trout (Oncorhynchus mykiss)
Journal of Limnology and Freshwater Fisheries Research, 4 (1) (2018), pp. 36-41
Wang et al., 2015
J.L. Wang, X.I. Meng, R.H. Lu, C. Wu, Y.T. Luo, X. Yan, et al.
Effects of Rehmannia glutinosa on growth performance, immunological parameters and disease resistance to Aeromonas hydrophila in common carp (Cyprinus carpio L.)
Aquaculture, 435 (2015), pp. 293-300, 10.1016/j.aquaculture.2014.10.004
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