Isolation, characterization and identification of extracellular enzyme producer Bacillus licheniformis from municipal wastewater and evaluation of their biodegradability
Nilesh Sonune, Anil Garode
Abstract
In the present investigation, a total of 34 bacterial isolates were isolated from municipal wastewater and sludge samples. Out of 34, 5 isolates were selected based on their growth on wastewater agar medium and were identified on the basis of morphological, cultural and biochemical tests. The enzyme production study showed that all the isolates were able to produce lipase and protease while one isolate (NW1D) showed amylase production. The bioremediation potential of these enzymes producing bacteria was investigated and the results suggest that the isolate NW6 was most efficient for bioremediation and showed reduction in biochemical oxidation demand (BOD), chemical oxidation demand (COD), nitrate and phosphate by 50.65%, 20%, 56.25% and 31.13% respectively after 72 h. Further, the NW6 isolate identified as Bacillus licheniformis by 16S rRNA sequencing and was optimized for different parameters to achieve effective results. The results showed that overall optimum inoculum size, retention time and agitation speed for NW6 were 10% (v/v), 96 h and 200 rpm respectively. The reduction in BOD, COD, nitrate and phosphate was found to be 54.55%, 36%, 24.83% and 26.42% respectively with 10% inoculum size. At 96 h retention time with 10% inoculum, BOD, COD, nitrate and phosphate reduction was 69.16%, 42.5%, 49.89% and 39.62% respectively. The reduction in BOD, COD, nitrate and phosphate was found to be 72.08%, 51%, 51.87% and 31.13% respectively at 200 rpm with 10% inoculum. Further, the NW6 may find application in municipal wastewater treatment to prevent water pollution as well as a potential enzyme production.
Keywords
References
Agarwal and Rajwar, 2010
A.K. Agarwal, G.S. Rajwar
Physico-chemical and microbiological study of Tehri Dam Reservoir Garhwal Himalaya
Journal of American Science, 6 (6) (2010), pp. 65-71
Ahring et al., 2001
B.K. Ahring, A.A. Ibrahim, Z. Mladenovska
Effect of temperature increase from 55 to 65 °C on performance and microbial population dynamics of an anaerobic reactor treating cattle manure
Water Research, 35 (2001), pp. 2446-2452
Aiswarya and Anu, 2017
M. Aiswarya, N. Anu
Bioremediation of ayurvedic hospital wastewater using Bacillus Cereus: Effect of agitation speed and time
International Journal for Research in Technological Studies, 4 (6) (2017), pp. 64-66
APHA, 2005
American Public Health Association (APHA), American Water Works Association (AWWA) and Water Environment federation (WEF)
Standard methods for the examination of water and wastewater
(21th ed.), APHA, Washington, DC (2005)
Bestawy et al., 2014
E.E. Bestawy, A. AL-Hejin, R. Amer, R.A. Kashmeri
Decontamination of domestic wastewater using suspended individual and mixed bacteria in batch system
Journal of Bioremediation and Biodegradation, 5 (5) (2014), p. 231, 10.4172/2155-6199.1000231
Claxton and Houx, 1995
L.D. Claxton, U.S. Houx
Integration of complex mixture toxicity and microbial analysis for environmental remediation research
Ecotoxicity and human health, Lewis Publishers (1995), pp. 467-468
Gaikwad et al., 2014
G.L. Gaikwad, S.R. Wate, D.S. Ramteke, K. Roychoudhury
Development of microbial consortia for the effective treatment of complex wastewater
Journal of Bioremediation and Biodegradation, 5 (4) (2014), p. 227, 10.4172/2155-6199.1000227
Garcha et al., 2014
S. Garcha, S.K. Brar, K. Sharma
Performance of laboratory prepared microbial consortium for degradation of dairy wastewater in batch system
Journal of Scientific and Industrial Research, 73 (2014), pp. 346-350
Garcha et al., 2016
S. Garcha, N. Vermab, S.K. Brar
Isolation, characterization and identification of microorganisms from unorganized dairy sector wastewater and sludge samples and evaluation of their biodegradability
Water Resources and Industry, 16 (2016), pp. 19-28
Garode and Sonune, 2013
A.M. Garode, N.A. Sonune
Isolation and identification of amylase producing bacterium from domestic wastewater of Buldana District (M.S.)
International Journal of Scientific Research, 2 (5) (2013), pp. 11-12
Garode and Sonune, 2014
A.M. Garode, N.A. Sonune
Screening and identification of lipase producing bacteria from domestic wastewater
International Journal of Pharma and Biosciences, 5 (2) (2014), pp. 265-268
Garode and Sonune, 2015
A.M. Garode, N.A. Sonune
Bacteriological and physico-chemical assessment of municipal wastewater from Buldana District, India
International Journal of Current Microbiology and Applied Sciences, 4 (7) (2015), pp. 577-584
Hamza et al., 2012
U.D. Hamza, I.A. Mohammed, A. Sale
Potentials of bacterial isolates in bioremediation of petroleum refinery wastewater
Journal of Applied Phytotechnology in Environmental Sanitation, 1 (3) (2012), pp. 131-138
Holt, 1994
J.G. Holt
Bergey's manual of determinative bacteriology
(9th ed.) (1994)
Janczukowicz et al., 2007
W. Janczukowicz, M. Zielinski, M. Debowski
Biodegradability evaluation of dairy effluents originated in selected sections of dairy production
Bioresource Technology, 99 (2007), pp. 4199-4205
Krishnaswamy et al., 2011
U. Krishnaswamy, M. Muthuchamy, L. Perumalsamy
Biological removal of phosphate from synthetic wastewater using bacterial consortium
Iranian Journal of Biotechnology, 9 (1) (2011), pp. 37-49
Kumari et al., 2008
S. Kumari, A. Mehta, S. Shukla, J. John, P. Mehta
Biodegradation potential of the selected bacterial strains isolated from Sagar Lake
Asian Journal of Experimental Sciences, 22 (1) (2008), pp. 43-48
Loos et al., 2013
R. Loos, R. Carvalho, D.C. Antonio, S. Comero, G. Locoro, S. Tavazzi, et al.
EU-wide monitoring survey on emerging polar organic contaminants in wastewater treatment plant effluents
Water Resources, 47 (17) (2013), pp. 6475-6487
Manh, 2008
L.D. Manh
Bioremediation of vegetable oil and grease from polluted wastewater in dairy factory
VNU Journal of Science, Natural Sciences and Technology, 24 (2008), pp. 56-62
Nimnoi et al., 2010
P. Nimnoi, N. Pongsilp, S. Lumyong
Genetic diversity and community of endophytic actinomycetes within the roots of Aquilaria crassna Pierre ex Lec assessed by actinomycetes-specific PCR and PCR-DGGE of 16S rRNA gene
Biochemical Systematics and Ecology, 38 (2010), pp. 595-601
Odeyemi et al., 2013
A.T. Odeyemi, B.I. Aderiye, O.S. Bamidele
Lipolytic activity of some strains of Klebsiella, Pseudomonas and Staphylococcus Spp. from restaurant wastewater and receiving stream
Journal of Microbiology Research, 3 (1) (2013), pp. 43-52
Ordaz-Diaz et al., 2014
L.A. Ordaz-Diaz, J.A. Rojas-Contreras, O.M. Rutiaga-Quinones, M.R. Moreno-Jimenez, F. Alatriste-Mondragon, S. Valle-Cervantes
Microorganism degradation efficiency in BOD analysis formulating a specific microbial consortium in a pulp and paper mill effluent
Bioresources, 9 (4) (2014), pp. 7189-7197
Pokhrel et al., 2013
B. Pokhrel, P. Wanjare, S. Singh, B. Purushotham, Kumara, M. Swamy
Isolation, screening and characterization of promising α-amylase producing bacteria from sewage enriched soil
International Journal of Advanced Biotechnology and Research, 4 (2) (2013), pp. 286-290
Ravi Kumar et al., 2013
B. Ravi Kumar, M. Lakshmi Prasad, D. Srinivasa Rao, K.R.S. Sambasiva Rao
Bioremediation of sewage using specific consortium of microorganisms
International Journal of Research in Applied, Natural and Social Sciences, 1 (6) (2013), pp. 15-26
Saha and Santra, 2014
A. Saha, S.C. Santra
Isolation and characterization of bacteria isolated from municipal solid waste for production of industrial enzymes and waste degradation
Journal of Microbiology and Experimentation, 1 (1) (2014), p. 00003, 10.15406/jmen.2014.01.00003
Sinha and Paul, 2014
S.N. Sinha, D. Paul
Biodegradation potential of some bacterial strains isolated from sewage water
International Journal of Environmental Biology, 4 (2) (2014), pp. 107-111
Sonune and Garode, 2015
N.A. Sonune, A.M. Garode
Bioremediation potential of bacterial isolates for municipal wastewater treatment
Current World Environment, 10 (2) (2015), pp. 619-625
Sonune et al., 2015
N.A. Sonune, N.A. Mungal, S.P. Kamble
Study of physico-chemical characteristics of domestic wastewater in Vishnupuri, Nanded, India
International Journal of Current Microbiology and Applied Sciences, 4 (1) (2015), pp. 533-536
Tamil selvi et al., 2012
A. Tamil selvi, E. Anjugam, R. Archana Devi, B. Madhan, S. Kannappan
Isolation and characterization of bacteria from tannery effluent treatment plant and their tolerance to heavy metals and antibodies
Asian Journal of Experimental Biological Sciences, 3 (2012), pp. 34-41
Usharani et al., 2009
U. Krishnaswamy, M. Muthusamy, L. Perumalsamy
Studies on the Efficiency of the Removal of Phosphate Using Bacterial Consortium for the Biotreatment of Phosphate Wastewater
European Journal of Applied Sciences, 1 (1) (2009), pp. 6-15
Vasconcellos et al., 2009
S.P. Vasconcellos, M.P. Cereda, J.R. Cagnon, M.A. Foglio, R.A. Rodrigues, G.P. Manfio, et al.
In vitro degradation of linamarin by microorganism isolated from cassava wastewater treatment lagoons
Brazilian Journal of Microbiology, 40 (2009), pp. 879-883
Verma and Baiswar, 2013
T. Verma, V. Baiswar
Isolation and characterization of extracellular thermoalkaline protease producing Bacillus cereus isolated from tannery effluent
International Journal of Engineering and Science, 2 (7) (2013), pp. 23-29
Wayne, 2009
P.A. Wayne
Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard
(8th ed.), M07-A8 Clinical and Laboratory Standards Institute (2009)
Wilson and Carson, 2001
T. Wilson, J. Carson
Rapid, high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media
Letters in Applied Microbiology, 32 (2001), pp. 326-330
Zhao et al., 2009
S. Zhao, N. Hu, Z. Chen, B. Zhao, Y. Liang
Bioremediation of reclaimed wastewater used as landscape water by using the denitrifying bacterium Bacillus cereus
Bulletin of Environmental Contamination and Toxicology, 83 (2009), pp. 337-340
Facebook
Google+
Twitter
LinkedIn
Mendeley
StumbleUpon
CiteULike
Reddit
Email